The ‘Microbiology and Immunology’ project will provide ‘state of the art’ genomic mapping of pigs and poultry in intensive farming systems. The project will study changes in the intestinal biota, immune status and whole genome of animals, in different farming environments and with different disease status, from many sites across the European Union. This project consists of 3 partners:
(1) Professor Ivan Rychlik and his team (Veterinary Research Institute, Czech Republic) will analyse changes in the intestinal microbiota of pigs and chickens in different farming environments, using Next generation sequencing. This will compare variation within bacterial RNA sequences, which will determine changes in species dynamics.
(2) Dr Philippe Velge’s team at INRA (France) will utilise high through-put PCR to examine changes in expression of 90 pig and chicken immune genes. Samples from INRA will also be sent to University of Nottingham for genomic analysis.
(3) Dr Neil Foster’s team (University of Nottingham) will use DNA microarray analysis to measure the expression of 45,000 genes within pig and chicken tissues. Pathway analysis will then be used to determine the interaction of these genes. It is envisaged that the milestone achieved within this PROHEALTH project may lead to novel diagnostic biomarkers of production disease in pigs and chickens.